How to determine the specificity of an antibody for Western Blot
The Western blot application combines the resolving power of polyacrylamide gel electrophoresis, the specificity of antibodies, and the sensitivity of enzyme assays. Although every step of a Western blot procedure must be carefully considered, fundamental to its success is the ability of the primary antibody to detect the protein of interest. But how to validate an antibody for Western blot?
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