How to determine the specificity of an antibody for Western Blot

January 29, 2020

The Western blot application combines the resolving power of polyacrylamide gel electrophoresis, the specificity of antibodies, and the sensitivity of enzyme assays. Although every step of a Western blot procedure must be carefully considered, fundamental to its success is the ability of the primary antibody to detect the protein of interest. But how to validate an antibody for Western blot?

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Antibody Validation / Western Blot

How to achieve greater separation in Western blot when proteins have a similar size

January 14, 2020

Despite its overall simplicity, the Western Blot application is a powerful procedure for the immunodetection of proteins and an essential tool within biological research. Nevertheless, proteins of the same length or similar size, such as isoform of the same protein, are difficult to separate. With some adjustments you can however achieve great results. Here we share some tips.

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Western Blot

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