Enhanced Validation of primary antibodies: what it is and how it helps your research get published.

November 03, 2020

Imagine buying a new antibody from a company for the very first time. You know nothing about the brand, the quality of the product, or dedication to customer satisfaction.

Like most researchers, you’d search for information on the vendor’s website, reviews from past customers, looking for any proof that the antibody will meet your needs. Don’t waste too much time looking around.

Come prepared! Do you know what to expect from a good antibody? And how and when it meets your expectations?

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Topics:

Antibody Validation

Interview with a scientist: traffic jams at the nuclear pores.

October 06, 2020

Dysfunctional nuclear-cytoplasmic trafficking of RNA binding proteins might contribute to neurological disorders. For this blog post we spoke with Dr. Rita Sattler.She employs human patient-derived induced pluripotent stem cells to elucidate the nuclear-cytoplasmic trafficking mechanisms in neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD).

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Antibody Validation / Immunohistochemistry / interview / atlasantibodies

Interview with a scientist: drug repurposing in oncology

August 18, 2020

Drug repurposing is the strategy of using existing drugs originally developed for a specific disease to treat other diseases. Today we spoke with Dr. Joanna Zawacka-Pankau, PhD in biochemistry. We asked her about her research efforts in drug repurposing to treat cancer.

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Antibody Validation / Oncology / interview / atlasantibodies / drugrepurposing

Microglia: the brain’s immune barrier at the interface of good and bad

April 21, 2020

Microglia are brain-resident macrophages forming the first active immune barrier in the central nervous system. Microglia are endowed with a spectacular plasticity, allowing them to acquire multiple phenotypes and thereby fulfill their numerous functions in health and disease. In this blog post we share an overview of the existing knowledge regarding the neuro-modulatory function of microglia. 

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Topics:

Antibody Validation / Human Protein Atlas / Immunohistochemistry

How to determine the specificity of an antibody for Western Blot

January 29, 2020

The Western blot application combines the resolving power of polyacrylamide gel electrophoresis, the specificity of antibodies, and the sensitivity of enzyme assays. Although every step of a Western blot procedure must be carefully considered, fundamental to its success is the ability of the primary antibody to detect the protein of interest. But how to validate an antibody for Western blot?

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Antibody Validation / Western Blot

Happy one-year blog anniversary to us! Recap of our most popular posts

December 10, 2019

This month we celebrate the first anniversary of our blog. It has been an incredible journey. If you have missed our blog posts, read on. We have interviewed scientists, discussed the importance of antibodies validation, the human protein atlas project and we gave you advice on how to succeed with your immunohistochemistry.

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Topics:

Antibody Validation / Human Protein Atlas / Immunohistochemistry / happybirthday / interview

3 case studies: antibodies are valuable tools in clinical research

October 01, 2019

Applied biological or biomedical research aims to reach a thorough understanding of the physiology of organisms and to further development of disease therapeutics. Some scientific discoveries have narrow applicability or scope and are sometimes serendipitous or even entirely unexpected. Other have wider applicability and transferability having proved enormously useful over a wide range of domains. Among the latter are research antibodies. Read more to discover how they help progressing science.

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Antibody Validation / Immunohistochemistry

Contribute to advancing science by publishing your negative results

August 20, 2019

We have run the exact same experiment. I found X. You found Y. Why? Have you been in this conversation with a collaborator? Have you ever obtained amazing results which contradict previous theory? Before you read on, take a moment to answer that for yourself. What was your reaction? “It is all my fault. I must have done something wrong” or “Interesting, I never thought about that before…” 

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Antibody Validation

Can I use an antibody targeting a human protein in another model organism?

May 14, 2019

Can you make use of an antibody that targets a human protein in another model organism? Under the right conditions, the answer is yes! 

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Topics:

Antibody Validation

Control your IHC: primary antibody controls you should know

February 19, 2019

How many decisions do you think you make every day? Would you be surprised if I tell you that an adult person makes about 25,000 more or less conscious decisions every day? It’s a fact. Consciously or unconsciously, we spend every day of our lives making decisions: where to go, what to wear, what to eat, how to spend our time, etc. But how much time do you spend making a decision for the right control for your primary antibody? And, most importantly, did you make the right choice? How can you tell a good decision from a bad one?

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Antibody Validation / Immunohistochemistry

How do I know if I can trust my antibody?

January 08, 2019

Have you heard of The Domino Effect? I’m sure you have but let me remind you what it is. The Domino Effect is the cumulative, often disastrous, effect produced when one single event sets off a chain of other events, the last one depending on the very first one.

A ‘domino effect’ is not merely a phenomenon that just happens to you, but something you can control and drive. It is in your power to pick the cards that lead towards success. So why not to start with an antibody that you can trust?

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Topics:

Antibody Validation

Journals require validated antibodies. What does that mean for me?

November 19, 2018

Antibody validation is critical to reproducibility and a necessary step to increase the security of antibody specificity in every specific context relating the target protein, sample, and application. Yet all too often, antibody-based published data cannot be reproduced. If you want your scientific project to be taken seriously, you should spend some time upfront to validate your antibodies before starting the project. Read on to know why this is so important.

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Antibody Validation

5 tips about validation when choosing an antibody

November 19, 2018

Say you’re in the lab, on your computer, searching for an antibody. After mining the data from the resulting loads of possibilities, your choice falls among 2 or 3 candidates. Great. But which one is the right one for you? Is it a complicated decision, or a simple one? Finding an antibody that works for your specific application can be a difficult task. Dozens of companies sell antibodies against your target proteins, and with so much choice available, looking for the perfect one can sometimes feel like a never-ending search. Read on. Here we share our 5 key points to ask yourself when choosing your next antibody.

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Topics:

Antibody Validation

Enhanced Validation of primary antibodies: what it is and how it helps your research get published.

November 19, 2018

Imagine buying a new antibody from a company for the very first time. You know nothing about the brand, the quality of the product, or dedication to customer satisfaction.

Like most researchers, you’d search for information on the vendor’s website, reviews from past customers, looking for any proof that the antibody will meet your needs. Don’t waste too much time looking around.

Come prepared! Do you know what to expect from a good antibody? And how and when it meets your expectations?

Learn More

Topics:

Antibody Validation

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