Headphones on, I am listening to my favorite music and staring at the lab bench in front of me feeling excited to start the immunohistochemistry experiment of the day. Pipette, timer, wells, vials, brush, buffers. Protocol taped on the shelf. Everything is ready. I feel like a DJ at the console, and I find myself thinking: what makes a good DJ? A DJ does not just play one song after the other in a random sequence. He has everything under control, he plans and underpins, every minute of a performance to varying degrees. So, how do I get started when planning a new set for a forthcoming immunohistochemistry experiment? Read on to find out how to control your results and prepare for a successful ’gig’ in the lab.