Ciliary motility is essential for moving fluids and particles over epithelial surfaces. Frequently, the limitations of quantitative tests and the lack of awareness delay the diagnosis of cilia diseases. In this blog post, we present antibodies- and 3D imaging-based quantitative super-resolution toolbox for the diagnosis of primary ciliary dyskinesia, a disorder of motile cilia. The researchers took part in our Explorer Program. Read on to discover what they found.
Cilia are slender, microscopic, highly conserved hair-like structures or organelles that line the surfaces of individual cells of nearly all mammalian cells. Cilia beat in rhythmic waves and use sweeping motions, moving liquids along internal epithelial tissue in animals. Recent discoveries have revealed that cilia have crucial roles in the signal transduction pathways that regulate intracellular calcium levels and maintain cellular homeostasis. The dysfunction of cilia causes diseases known as ciliopathies.
Motile and non‐motile cilia
There are two types of cilia: motile and non-motile cilia. The non-motile cilia (also called primary cilia) typically serve as sensory organelles. Whereas primary cilia have relatively little additional structure, motile cilia have both a central doublet of microtubules as well as inner and outer dynein arms and radial spokes, which are all needed for motility.
There is a third type of cilium, known as nodal cilia, but this is only transiently present in the early embryo and is crucial in the establishment of the left-to right body asymmetry.
Figure. 1. Illustration of the motile cilia structure. A basal body and an axoneme composed the cilia. The cilia axoneme is the core and the most prominent structural component of the cilia. TZ, transition zone; DA, distal appendage; BF, basal foot (Liu Z. et al, 2020).
Motile' cilia are found in the lungs, respiratory tract, and middle ear. Airway clearance of pathogens and particulates relies on motile cilia. These cilia have a rhythmic waving or beating motion. For instance, they work to keep the airways clear of mucus and dirt, allowing us to breathe easily and without irritation. Impaired cilia motility is associated with recurrent infections, leading to a reduction in lung function (bronchiectasis), lung transplant, or death in some cases.
Non-motile cilia are found in the eye, inside the light-sensitive cells (photoreceptors) of the retina and in most cells of the body that are not dividing (with few exceptions). These cilia act like microscopic train-tracks and allow the transport of vital molecules from one end of the photoreceptor to the other. In the kidney, non-motile cilia bend with urine flow and send a signal to alert the cells that there is a flow of urine.
Ciliopathies are inherited human disorders that affect primary cilia genes. They are caused by both motile and non‐motile cilia dysfunction and are becoming a rapidly expanding disease category. The definition of ciliopathies is evolving while we understand more about them.
At least 35 different ciliopathies, collectively affect nearly all organ systems, with prevalent phenotypes including polycystic kidney disease, retinal degeneration, obesity, skeletal malformations, and brain anomalies.
The genetic heterogeneity, together with the high phenotypic variability, overlapping phenotypes, and the progressive onset of many features during childhood and adolescence, complicates both the clinical and molecular diagnosis of ciliopathies.
Genetic tools such as next-generation sequencing, high-throughput technologies, and advanced bioinformatics tools developed in the last decade are a powerful way of improving our understanding of the biological basis of ciliopathy disorders.
|Figure 2. Schematic showing rotational polarity in cells from healthy individuals and patients with PCD (Liu et al 2020).|
A need for quantitative methods for improving PCD diagnosis
Primary ciliary dyskinesia (PCD) is an autosomal recessive genetic condition in which the motile cilia in the respiratory system are defective. PCD affects how cilia grow and function (Figure 2).
Disease caused by proteins functioning in motile cilia can cause different phenotypes such as infertility, cardiac malformation, situs inversus, and lung disease. PCD refers mostly to the lung phenotype.
PCD is a disorder characterized by chronic respiratory tract infections and abnormally positioned internal organs. Faulty genes cause the cilia to be immotile, stiff, uncoordinated, and sometimes in the wrong size or shape. Sometimes the cilia are missing altogether. If the cilia don't work well, bacteria stay in the airways, causing breathing problems, infections, and other disorders.
The challenge: from a clinical to a molecular diagnosis
The clinical diagnosis of PCD symptoms like cough and rhinitis is not specific enough for an accurate diagnosis. For this reason, besides the clinical diagnosis, a precise quantitative molecular determination is essential for identifying clear targets for future therapies or for initiating treatments that can stabilize lung function.
However, to date, the molecular diagnosis of PCD has mainly relied on non-quantitative methods with limited sensitivity or that require a priori knowledge of the genes involved.
The generation of the PCD proteome map
Over 50 proteins regulating cilia motility are linked to PCD, and more are awaiting validation. To improve the diagnosis of PCD, Liu, and colleagues from the University of Toronto, Canada, generated a PCD imaging proteome map and developed a toolbox of quantitative imaging methods.
The study, published in Science Translational Medicine, 2020:12(535), uses a combination of imaging approaches that produce quantitative results useful to improve upon current, non-quantitative diagnostic methods.
The new super-resolution based imaging methods comprise antibodies immunoassays combined with 3D structured illumination microscopy (3D-SIM) and stochastic optical reconstruction microscopy (STORM). Super-resolution microscopy on nasal airway cells from PCD patients can identify proteins in the wrong locations, defects in cellular structure, and impaired beating movements in the cilia.
The goals of this quantitative super-resolution microscopy workflow are:
- to increase sensitivity and throughput.
- to detect structural defects in PCD patients’ cells.
- to quantify motility defects caused by yet to be found PCD genes.
Now I see you, cilia!
A panel of 21 commercially available antibodies directed against PCD proteins was validated to detect molecular defects in multiciliated airway cells from patients with PCD. Ciliated epithelium was obtained by nasal brush biopsy.
The table below lists some antibodies from Atlas Antibodies used in the study.
|Product name||Catalogue Nr||Product name||Catalogue Nr|
After immunostaining, the isolated multiciliated cells were imaged by 3D-SIM or STORM, a technique able to achieve ~25-nm in-plane and ~50-nm axial resolution, and which allows detailed visualization of distributions of specific PCD proteins thus increasing the final image resolution (Figure 3).
Figure 3. Comparison between STORM imaging and conventional fluorescence imaging. STORM imaging of GAS8 with the anti-GAS8 polyclonal antibody (HPA041311) is visible in green. Insets in white boxes are high-magnification images of three ciliary regions showing periodic distribution. Box on the far-right shows high-magnification image of an intact cilium from the STORM image on the left (Liu et al 2020).
- Primary ciliary dyskinesia (PCD) is a genetic condition in which cilia in the respiratory system are malfunctioning.
- The diagnosis of PCD is frequently delayed until well after bronchiectasis has developed.
- PCD diagnosis relies on nonquantitative methods with limited sensitivity.
- The new super-resolution imaging method published by Liu et al., 2020 comprises antibodies immunoassays combined with structured illumination microscopy (3D-SIM) and stochastic optical reconstruction microscopy (STORM).
- 3D-SIM/STORM is a sensitive, simple, and powerful tool for improving the diagnosis of motile ciliopathies and can integrate or replace some current clinical diagnostic methods.
- Together, this data show that super-resolution methods are powerful tools for improving diagnosis of motile ciliopathies.
Zhen Liu, Quynh P. H. Nguyen, Qingxu Guan, Alexandra Albulescu, Lauren Erdman, Yasaman Mahdaviyeh, Jasmine Kang, Hong Ouyang, Richard G. Hegele, Theo Moraes, Anna Goldenberg, Sharon D. Dell, Vito Mennella.
A quantitative super-resolution imaging toolbox for diagnosis of motile ciliopathies.
Science Translational Medicine, 2020:12(535)