Dr. Laura Pozzi

Dr. Laura Pozzi is a scientific writer at Atlas Antibodies. She holds a Ph.D. in Life and Biomolecular Science from the Open University of London in collaboration with the Mario Negri Institute for Pharmacological Research in Milan. Laura has worked as a researcher at Karolinska Institutet in Sweden and more recently as associated editor. She has a long track record of scientific publications as a first author and as coauthor. Her research focus on neuroscience with a broad experience in antibodies validation and immunohistochemistry techniques.

Recent Posts

How to achieve greater separation in Western blot when proteins have a similar size

January 14, 2020

Despite its overall simplicity, the Western Blot application is a powerful procedure for the immunodetection of proteins and an essential tool within biological research. Nevertheless, proteins of the same length or similar size such as isoforms of the same protein, are difficult to separate. With some adjustments you can however achieve great results. Here we share some tips.

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Topics:

Western Blot

Happy one-year blog anniversary to us! Recap of our most popular posts

December 10, 2019

This month we celebrate the first anniversary of our blog. It has been an incredible journey. If you have missed our blog posts, read on. We have interviewed scientists, discussed the importance of antibodies validation, the human protein atlas project and we gave you advice on how to succeed with your immunohistochemistry.

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Topics:

Antibody Validation / Human Protein Atlas / Immunohistochemistry

Interview with a scientist: Cell Atlas - past, present and future challenges

November 26, 2019

Today we meet with Dr. Peter Thul, group leader at the Human Protein Atlas (HPA) project, Stockholm, Sweden. Peter is one of the scientists behind the Cell Atlas: a database that provides high-resolution insights into the expression and spatio-temporal distribution of proteins within human cells. We asked Peter 10 questions about the past, the present, and the future of the Cell Atlas.

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Topics:

Human Protein Atlas

Free webinar - The Brain Atlas: A road map through the complex protein signature of the brain

November 12, 2019

Attend our AAAS/Science-hosted webinar “The Brain Atlas: A road map through the complex protein signature of the brain” and learn more about the Brain Atlas, part of the Human Protein Atlas (HPA), from the scientists behind the project. Register today!

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Topics:

Human Protein Atlas

Interview with a scientist: Brain Atlas - past, present and future challenges

October 29, 2019

Today we meet with Dr. Jan Mulder, group leader of the Human Protein Atlas (HPA) brain profiling group at Karolinska Institutet, Stockholm, Sweden, and the director of the Brain Atlas, a brand-new addition to the (HPA) project. His research interest is to identify proteins involved in brain development, normal brain physiology and pathophysiology of brain disorders by using an antibody-based approach combined with multiplex fluorescence immunohistochemistry.

We asked Jan 10 questions about the past, the present, and the future of the Brain Atlas.

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Topics:

Human Protein Atlas / Immunohistochemistry

Mapping the human brain - a guide to the Brain Atlas

October 15, 2019

The Brain Atlas is a tool that brings the human brain mapping on a new level. It provides an overview of all the proteins expressed in the brains of three mammalian species: human, mouse and pig. It also compares the proteins in the brain to the proteins in other tissue types of the human body. Excited? Read more.

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Topics:

Human Protein Atlas

3 case studies: antibodies are valuable tools in clinical research

October 01, 2019

Applied biological or biomedical research aims to reach a thorough understanding of the physiology of organisms and to further development of disease therapeutics. Some scientific discoveries have narrow applicability or scope and are sometimes serendipitous or even entirely unexpected. Other have wider applicability and transferability having proved enormously useful over a wide range of domains. Among the latter are research antibodies. Read more to discover how they help progressing science.

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Topics:

Antibody Validation / Immunohistochemistry

Buffers and chemicals - how to succeed with your IHC part III

September 17, 2019

Washing- and staining solutions, blocking agents, detection and mounting solutions. Successful immunohistochemical staining requires reliable, high-quality reagents to fixate, to wash and permeabilize the tissue. But what is the rationale behind the fact that certain buffers or storage conditions are better to use than other, or is it all just empiric?

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Topics:

Immunohistochemistry

Interview with a scientist: Tissue Atlas - past, present and future challenges

September 03, 2019

Today we meet with Dr. Cecilia Lindskog, group leader at Uppsala University in Sweden, and the site director of the Tissue Atlas part of the Human Protein Atlas (HPA). Her research is focused on protein science, understanding the biology and functions of human proteins expressed in different organs, and the underlying mechanisms leading to cancer and other diseases. We asked her 10 questions about the past, the present, and the future of the Tissue Atlas.

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Topics:

Human Protein Atlas / Immunohistochemistry

Contribute to advancing science by publishing your negative results

August 20, 2019

We have run the exact same experiment. I found X. You found Y. Why? Have you been in this conversation with a collaborator? Have you ever obtained amazing results which contradict previous theory? Before you read on, take a moment to answer that for yourself. What was your reaction? “It is all my fault. I must have done something wrong” or “Interesting, I never thought about that before…” 

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Topics:

Antibody Validation

Which cells or organelles are my proteins expressed in? An overview using the Cell Atlas

July 23, 2019

The human body is the results of trillion of cells communicating with each other. Early biologists described cells as simple membranous sacs containing fluid and a few floating particles. Today we know that cells are infinitely more complex than that. Read here about the Cell Atlas, part of the Human Protein Atlas, for insights into the spatio-temporal distribution of proteins within human cells. 

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Topics:

Human Protein Atlas

Interview with a scientist: multiplex IHC - past, present and future challenges

May 28, 2019

Today we meet with Dr. Kristian Moller, Principal Scientist at Altas Antibodies, to ask him 10 questions about past, present, and future of multiplex immunohistochemistry.

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Topics:

Immunohistochemistry

Can I use an antibody targeting a human protein in another model organism?

May 14, 2019

Can you make use of an antibody that targets a human protein in another model organism? Under the right conditions, the answer is yes! 

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Topics:

Antibody Validation

Interview with a scientist: IHC - past, present and future challenges

April 30, 2019

Today we meet with Dr. Caroline Kampf to ask her 10 questions about past, present, and future of immunohistochemistry (IHC). 

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Topics:

Immunohistochemistry

Antigen retrieval - how to succeed with your IHC part II

April 16, 2019

How many samples? How much money and time have you wasted trying to unmask a protein on your IHC samples? Tissue fixation in immunohistochemistry often negatively impacts on your immunostaining by masking the epitope of interest. Read on to learn about the most common antigen retrieval methods used to restore epitope-antibody binding.

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Topics:

Immunohistochemistry

Artifacts in IHC: the usual suspects - part II

March 19, 2019

 “I don’t know why I am doing this. I can’t handle it!”  You thought you knew how to recognize and solve your IHC artifacts but after reading the troubleshooting guide the answers seem so far away. Long lists of tips. Examples too difficult to understand. Not-so-friendly images. Feeling overwhelmed? You are not alone. Although sometimes troubleshooting seems unbearable, there is no need to worry! Troubleshooting is nothing more than the logical elimination of variables.

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Topics:

Immunohistochemistry

Artifacts in IHC: the usual suspects - part I

March 19, 2019

After a few intense days of meticulous tissue washes and antibody incubations my staining is finally ready. I am staring at it down the microscope. I enjoy what I see: a dark brown color exactly where I expect to see it! Am I good or what? I give myself a figurative pat on the back and an imaginary high five. Excited and proud, I call my supervisor who glares intently at it and then firmly, and rather surprisingly, asks me to re-run the staining. What went wrong? What did I miss? I must get to the bottom of this!

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Topics:

Immunohistochemistry

How do I know if I can trust my antibody?

January 08, 2019

Have you heard of The Domino Effect? I’m sure you have but let me remind you what it is. The Domino Effect is the cumulative, often disastrous, effect produced when one single event sets off a chain of other events, the last one depending on the very first one.

A ‘domino effect’ is not merely a phenomenon that just happens to you, but something you can control and drive. It is in your power to pick the cards that lead towards success. So why not to start with an antibody that you can trust?

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Topics:

Antibody Validation

5 tips about validation when choosing an antibody

November 19, 2018

Say you’re in the lab, on your computer, searching for an antibody. After mining the data from the resulting loads of possibilities, your choice falls among 2 or 3 candidates. Great. But which one is the right one for you? Is it a complicated decision, or a simple one? Finding an antibody that works for your specific application can be a difficult task. Dozens of companies sell antibodies against your target proteins, and with so much choice available, looking for the perfect one can sometimes feel like a never-ending search. Read on. Here we share our 5 key points to ask yourself when choosing your next antibody.

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Topics:

Antibody Validation

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